| Ukrainian Community in Barrie, Online/Web Service | Barrie |
| Ukrainian National Federation of Canada, Barrie Simcoe, UNF Barrie Simcoe | Barrie |
| United Association for Plumbers, Steamfitters and Welders, Barrie Office, Local 46 | Barrie |
| United Way Simcoe Muskoka, Eat Well to Excel - North Simcoe & South Simcoe | Midland |
| United Way Simcoe Muskoka, Simcoe County, Low-Income Energy Assistance Program (LEAP) | Springwater |
| United Way Simcoe Muskoka, Simcoe County | Springwater |
| Unity Christian High School | Barrie |
| University of Toronto, Comparative Education Service | Toronto |
| University of Toronto, Faculty of Dentistry, Dental Clinics | Toronto |
| UPlift Black Centre for Social Justice and Inclusion, Barrie | Barrie |
| Upper Canada Child Care, Alliston - Boyne River Public School, Child Care Centre | New Tecumseth |
| Upper Canada Child Care, Alliston - St Paul's Catholic School, Before and After School Child Care | New Tecumseth |
| Upper Canada Child Care, Alliston - Union Public School, Before and After School Child Care | New Tecumseth |
| Upper Canada Child Care, Alliston - Wellington St E, Child Care Centre | New Tecumseth |
| Upper Canada Child Care, Barrie - Assikinack Public School, Before and After School Child Care | Barrie |
| Upper Canada Child Care, Barrie - Emma King Public School, Before and After School Child Care | Barrie |
| Upper Canada Child Care, Barrie - Hyde Park Public School, Before and After School Child Care | Barrie |
| Upper Canada Child Care, Barrie - Johnson St Public School, Before and After School Child Care | Barrie |
| Upper Canada Child Care, Barrie - Portage View Public School, Before and After School Child Care | Barrie |
| Upper Canada Child Care, Barrie - St Michael the Archangel Catholic School, Before and After School Child Care | Barrie |
| Upper Canada Child Care, Barrie - Warnica Public School, Before and After School Child Care | Barrie |
| Upper Canada Child Care, Barrie - Willow Landing Public School, Child Care Centre | Barrie |
| Upper Canada Child Care, Cookstown Central Public School, Before and After School Child Care | Innisfil |
| Upper Canada Child Care, Everett - Tosorontio Central Public School, Before and After School Child Care | Adjala-Tosorontio |
| Upper Canada Child Care, Innisfil - Lake Simcoe Public School, Child Care Centre | Innisfil |
| Upper Canada Child Care, Innisfil - Sunnybrae Public School, Before and After School Child Care | Innisfil |
| Upper Canada Child Care, Oro Station - Guthrie Public School, Before and After School Child Care | Oro-Medonte |
| Uptown Boxing | Barrie |
| Urban Village Academy, Barrie | Barrie |